background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...

Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...

a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

genetic transformation studies were carried out to standardize a protocol for agrobacterium -mediated genetic transformation of three economically important strawberry (fragaria x ananassa duch) cultivars kurdistan’, camarosa’, and ‘paros’. shoot regeneration frequency 72, 65 and 30% was obtained on ms (1) basal medium supplemented with 2% glucose and 4 mg/l tdz for camarosa, kurdistan and paro...

Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...

background: bone morphogenetic protein 2 (bmp- 2) belongs to the tgf-β superfamily of proteins and plays an important role in the development of bone and cartilage. bmp- 2 is also associated with maintenance and repair of damaged bone. recombinant human bone morphogenetic protein 2 (rhbmp- 2) is now produced by genetic engineering techniques and used in treatment of thin bone fractures in the j...

in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

cotton (gossypium hirsutum l.)  is an important fiber crop in iran, cultivated on 150000-200000 ha of land.  in iran the estimated loss due to the insect pest is more than 30%. traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). in order to produce transgenic cotton resistance to in...

Immature embryos as a choice tissue for genetic transformation of maize have a few limitations, such as genotype dependence, time-consuming and requiring a well-equipped greenhouse for access, at any time. In the present study, the split-seed explants were used for genetic transformation of maize, B73 line. The transformation of maize split-seed explants from the inbred line B73, for resistance...